qpcr reactions (Roche)
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99
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qpcr reactions
Qpcr Reactions, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qpcr+reactions/LightCycler+480+System/pmc12859463-380-11-21
Average 99 stars, based on 6 article reviews
Qpcr Reactions, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qpcr+reactions/LightCycler+480+System/pmc12859463-380-11-21
Average 99 stars, based on 6 article reviews
qpcr reactions - by Bioz Stars,
2026-09
99/100 stars
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Related Articles
Real-time Polymerase Chain Reaction:Article Title: Role of MIR-101-3P/RAP1B Axis and Insulin Protection in Glucose-Induced Stress in HK-2 Cell Model for Diabetic Kidney Disease. Article Snippet: 1 Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia 2 Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia 3 Promoting Resilience and Innovation in Muldisciplinary Ageing Excellence (PrimAGE), Malaysian Research Institute on Ageing (MyAgeing®), Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia 4 Brain and Mental Health Research Advancement and Innovation Networks (PUTRA BRAIN), Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400 UPM, Selangor, Malaysia Abstract Proximal tubular cell (PTC) injury is a critical driver of diabetic kidney disease (DKD).. This study aimed to characterize the molecular response of HK-2 PTCs to acute glucotoxicity, focusing on the miR-101-3p/RAP1B axis, insulin-mediated protection, and the primary mechanisms of cell death.. HK-2 cells were exposed to varying glucose concentrations (5.5– 128 mM) with or without insulin (10 mg/L) for 48 h. We assessed cell viability, mitochondrial membrane potential (ΔΨm), and intracellular reactive oxygen species (ROS). Article Title: Transcriptome analysis of the prefrontal cortex identifies inflammatory genes associated with cognitive impairment in a model of multiple sclerosis. Article Snippet: .. The Article Title: Bioenzymatic single-cell microencapsulation for enhanced stem Cell therapy Article Snippet: RNA was eluted with 30 μL elution buffer incubated at room temperature for 2 min, followed by centrifugation at 12,000× g for 1 min. RNA concentration and purity (A260/A280 ratio) were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific, One/OneC) with elution buffer as blank. .. Purified RNA was reverse-transcribed into complementary DNA (cDNA) using reverse transcriptase. Article Title: The Ability of Probiotic Strain Escherichia coli O83:K24:H31 to Modulate Gut Homeostasis and Immune Function After Antibiotic-Induced Dysbiosis. Article Snippet: .. The Article Title: Metabolic Profiling and In Vitro Assessment of the Immunomodulatory Effects of Hydrodistillation-Derived Extracts from the Fruticose Lichen Pseudevernia furfuracea (L.) Zopf. on Human Lymphocytes. Article Snippet: Total RNA was extracted using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and any residual genomic DNA was eliminated using an RNase-Free DNase Set (Qiagen). .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA with a QuantiTect® Reverse Transcription Kit (Qiagen) and diluted 1:5 before amplification. Article Title: Spatiotemporal mapping reveals Ccl8 hi macrophages as key drivers of testicular inflammaging Article Snippet: Two micrograms of total RNA were reverse transcribed into cDNA using the PrimeScript RT Master Mix Kit (Cat#RR036A, Takara) with incubation at 37°C for 15 min followed by 85°C for 5 s. Quantitative PCR (qPCR) was performed using TB Green Premix Ex Taq II (Cat# RR820A, Takara) following the manufacturer's instructions. .. The Article Title: Transcription fidelity and control of alternative splicing contribute to heat stress survival in Arabidopsis Article Snippet: For RT-qPCR assays, 5 μg total RNA was treated with DNase I according to the manufacturer instructions (Ambion AM2222, www.thermofisher.com ), then precipitated in ethanol and resuspended in sterile water. .. 1 μg of DNase I-treated RNA and random primer was used for the first-strand cDNA reaction (NEB, E6300S, neb.com ). qPCRs were done using qPCR Master Mix (NEB, M3003S, neb.com ). Article Title: Discovery of a new allosteric inhibitor for human dopamine transporter by physics-based modeling and experimental validation. Article Snippet: The dopamine transporter (DAT) is a pivotal target in the management of central nervous system (CNS) disorders.. Existing drugs that competitively bind to the orthosteric site on DAT are associated with several side effects.. Although the concept of allostery in DAT has been acknowledged for years, the precise allosteric sites remain elusive, impeding the rational design of allosteric modulators for DAT. SYBR Green Assay:Article Title: Transcriptome analysis of the prefrontal cortex identifies inflammatory genes associated with cognitive impairment in a model of multiple sclerosis. Article Snippet: .. The Article Title: Bioenzymatic single-cell microencapsulation for enhanced stem Cell therapy Article Snippet: RNA was eluted with 30 μL elution buffer incubated at room temperature for 2 min, followed by centrifugation at 12,000× g for 1 min. RNA concentration and purity (A260/A280 ratio) were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific, One/OneC) with elution buffer as blank. .. Purified RNA was reverse-transcribed into complementary DNA (cDNA) using reverse transcriptase. Purification:Article Title: Bioenzymatic single-cell microencapsulation for enhanced stem Cell therapy Article Snippet: RNA was eluted with 30 μL elution buffer incubated at room temperature for 2 min, followed by centrifugation at 12,000× g for 1 min. RNA concentration and purity (A260/A280 ratio) were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific, One/OneC) with elution buffer as blank. .. Purified RNA was reverse-transcribed into complementary DNA (cDNA) using reverse transcriptase. Reverse Transcription:Article Title: Bioenzymatic single-cell microencapsulation for enhanced stem Cell therapy Article Snippet: RNA was eluted with 30 μL elution buffer incubated at room temperature for 2 min, followed by centrifugation at 12,000× g for 1 min. RNA concentration and purity (A260/A280 ratio) were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific, One/OneC) with elution buffer as blank. .. Purified RNA was reverse-transcribed into complementary DNA (cDNA) using reverse transcriptase. Article Title: Metabolic Profiling and In Vitro Assessment of the Immunomodulatory Effects of Hydrodistillation-Derived Extracts from the Fruticose Lichen Pseudevernia furfuracea (L.) Zopf. on Human Lymphocytes. Article Snippet: Total RNA was extracted using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and any residual genomic DNA was eliminated using an RNase-Free DNase Set (Qiagen). .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA with a QuantiTect® Reverse Transcription Kit (Qiagen) and diluted 1:5 before amplification. Synthesized:Article Title: Metabolic Profiling and In Vitro Assessment of the Immunomodulatory Effects of Hydrodistillation-Derived Extracts from the Fruticose Lichen Pseudevernia furfuracea (L.) Zopf. on Human Lymphocytes. Article Snippet: Total RNA was extracted using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and any residual genomic DNA was eliminated using an RNase-Free DNase Set (Qiagen). .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA with a QuantiTect® Reverse Transcription Kit (Qiagen) and diluted 1:5 before amplification. Amplification:Article Title: Metabolic Profiling and In Vitro Assessment of the Immunomodulatory Effects of Hydrodistillation-Derived Extracts from the Fruticose Lichen Pseudevernia furfuracea (L.) Zopf. on Human Lymphocytes. Article Snippet: Total RNA was extracted using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and any residual genomic DNA was eliminated using an RNase-Free DNase Set (Qiagen). .. Complementary DNA (cDNA) was synthesized from 1 μg of total RNA with a QuantiTect® Reverse Transcription Kit (Qiagen) and diluted 1:5 before amplification. |